Enantioselective LA production from whey by low acid tolerant LAB has been analyzed.
The D(−)/L(+) ratio depended on LAB strain used for whey fermentation.
The maximum production of LA from hydrolysed whey was achieved at a pH 4.4–4.5.
Prolonged fermentation induced the formation of D(−) lactic acid.
The sustainability of fermentative lactic acid production has been increased.