Direct detection of phenylephrine 3-O-sulfate in LS180 human intestinal cells using a novel hydrophilic interaction liquid chromatography (HILIC) assay
Direct simultaneous quantitation of phenylephrine and its sulfate metabolite. Hydrophilic analytes: novel sugaring-out liquid-liquid extraction method. Hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection. Validated assay applied to study kinetics of phenylephrine sulfation.