New versatile, elastomeric, degradable polymeric materials for medicine
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- 作者：Saad ; B. ; Neuenschwander ; P. ; Uhlschmid ; G.K. ; Suter ; U.W.
- 关键词：Polyesterurethane ; Degradation ; Osteoblasts ; Chondrocytes ; Macrophages ; ALP ; alkaline phosphatase ; BOC ; t-butyloxycarbonyl ; BSA ; bovine serum albumin ; DCC ; N ; N-dicyclohexylcarbodiimide ; DPHB-P ; dansyl-labelled PHB-P ; EDTA ; ethylenediaminetetraacetic acid
- 刊名：International Journal of Biological Macromolecules
- 出版年：1999
- 出版时间：June, 1999
- 年：1999
- 卷：25
- 期：1-3
- 页码：293-301
- 全文大小：325 K
文摘
The present investigation was focused on the cell compatibility of recently developed biodegradable polyesterurethane-foam (DegraPol-foam) to chondrocytes and osteoblasts. Both chondrocytes and osteoblasts, isolated from adult male rats, exhibited relatively high cell adhesion on DegraPol-foam. Scanning electron microscopy (SEM) showed that cells grew on the surface and into the open cell pores of the foam. Morphologically, cells found on the surface of the foam exhibited a flat cell appearance and built a confluent cell multilayer. In contrast, inside the foams cell showed rounded morphology building cell aggregates and cell islets. In addition, chondrocytes and osteoblasts proliferated on the DegraPol-foam and preserved their phenotype for up to 2 weeks. During degradation of these polymers, small crystalline particles of short-chain poly[(R)-3-hydroxybutyric acid] (Mn≈2300) (PHB-P) and lysine methyl ester are released. Therefore, lysine methyl ester and PHB-P, as possible degradation products of the polymer, are investigated here for their effects on macrophages and osteoblasts. Results obtained in the present study clearly indicate that macrophages and, to a lesser degree, osteoblasts have the ability to take up (phagocytose) PHB-P. At low concentrations, particles of PHB failed to induce cytotoxic effects or to activate macrophages. Osteoblasts showed only limited PHB-P phagocytosis and no signs of any cellular damage. At high concentrations of PHB-P, the cell viability of macrophages and to a lesser extent of osteoblasts was affected.