Protonophoric activity of alkylamino-NBD derivatives was found in lipid membranes.
NBD-based fluorescent uncouplers of oxidative phosphorylation were screened.
n-Octylamino-NBD proved to be an uncoupler in mitochondria.
Half-maximal uncoupling concentration of n-octylamino-NBD was 5 渭M.
NBD-based uncouplers showed a bell-shaped dependence on the alkyl chain length.