A fluorescence high throughput screening method for the detection of reactive electrophiles as potential skin sensitizers
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- 作者：Cristina Avontoa ; Amar G. Chittiboyinaa ; Diego Ruab ; Ikhlas A. Khana ; c ; ikhan@olemiss.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ACN ; acetonitrile ; AOP ; adverse outcome pathway ; BQ ; benzoquinone ; CDNB ; 1-chloro-2 ; 4-dinitrobenzene ; COUM ; coumarin ; Cys-DPRA ; cysteine-direct peptide reactivity assay ; DBN ; 1 ; 5-diazabicyclo[4.3.0]non-5-ene ; DCYA ; dansyl cysteamine ; (DCYA)2 ; dansyl cysteamine dimer ; DMSO ; dimethyl sulfoxide ; DPRA ; direct peptide reactivity assay ; ESI ; electron spray ionization ; HPLC ; high performance liquid chromatography ; HR-MS ; high resolution mass spectroscopy ; HTS ; high throughput screening ; LLNA ; local lym
- 刊名：Toxicology and Applied Pharmacology
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：289
- 期：2
- 页码：177-184
- 全文大小：709 K
文摘
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A novel fluorescence-based method to detect electrophilic sensitizers is proposed.
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A model fluorescent thiol was used to directly quantify the reaction products.
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A discussion of the reaction workflow and critical parameters is presented.
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The method could provide a useful tool to complement existing chemical assays.