Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair
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- 作者：Tony Gutschner1 ; tgutschner@mdanderson.org" class="auth_mail" title="E-mail the corresponding author ; Monika Haemmerle2 ; 3 ; Giannicola Genovese1 ; Giulio F. Draetta1 ; 4 ; 5 ; Lynda Chin1 ; 4 ; lchin@mdanderson.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：CRISPR ; cell cycle ; genome editing ; MALAT1 ; homologous recombination ; proteolysis ; synthetic biology
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：16 February 2016
- 年：2016
- 卷：14
- 期：6
- 页码：1555-1566
- 全文大小：1826 K
文摘
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Homology-directed repair (HDR) enables generation of site-specific Knockins
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HDR is restricted to S/G2 and competes with non-homologous end-joining repair
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Cas9-hGem(1/110), a cell-cycle-tailored genome-editing tool, is generated
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Cas9-hGem(1/110) increases the rate of HDR up to 1.87-fold compared to wild-type Cas9