Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test

详细信息    查看全文

• 作者：Xiang GUO ; Xing Fen YANG ; Ying YANG ; Raabe HANS ; Jing Heng CAI ; Jin Yu XUE ; Xiao Hua TAN ; Xiao Ping XIE ; Xi Kun XIONG ; Jun Ming HUANG
• 关键词：Isolated rabbit eye ; IRE ; Eye irritation ; Alternative testing ; Draize test
• 刊名：Biomedical and Environmental Sciences
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：25
• 期：3
• 页码：359-366
• 全文大小：473 K

文摘

Objective

This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.

Methods

IRE test was performed according to modifications of the in vitro toxicology (INVITTOX) Protocol No.85: Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM), and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.

Results

IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test ¦Ö²=51.314, P<0.001; McNemar P=0.261; Gamma=0.960, P<0.001; Kappa=0.843, P<0.001) and 26 cosmetic products (Fisher's Exact Test ¦Ö²=15.522, P<0.001; McNemar P=0.311; Gamma=0.967, P<0.001; Kappa=0.611, P<0.001).

Conclusion

IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.