Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies
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- 作者：Artem D. Rogacheva ; b ; rogachev@nioch.nsc.ru" class="auth_mail" title="E-mail the corresponding author ; Olga I. Yarovayaa ; b ; Sergey V. Ankova ; Mikhail V. Khvostova ; Tatyana G. Tolstikovaa ; b ; Andrey G. Pokrovskyb ; Nariman F. Salakhutdinova ; b
- 关键词：DBS ; dried blood spot ; CV ; coefficient of variation ; EMA ; European medicinal agency ; FDA ; food and drug administration ; IS ; internal standard ; LC&ndash ; MS/MS ; liquid chromatography coupled with tandem mass-spectrometry ; LLOQ ; lowest limit of quantitation ; LOD ; limit of detection ; MF ; matrix factor ; MRM ; multiple reaction monitoring ; QC ; quality control ; QCH ; quality control sample of high concentration ; QCL ; quality control sample of low concentration ; QCM ; quality control sample of medium conce
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：1036-1037
- 期：Complete
- 页码：136-141
- 全文大小：1016 K
文摘
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The dried blood spot (DBS) technique was used for sample preparation.
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Twenty microlitres of whole blood from animal are enough for analysis.
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Less than 2 min was needed for each analytical run.
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The method was validated according to the FDA and EMA guidelines for bioanalytical methods.
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The method suitability was demonstrated by a pharmacokinetic study in rats.