Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization
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- 作者：Shohei Shigetoa ; Kazuyuki Matsudaa ; kmatsuda@shinshu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Akemi Yamaguchib ; Akane Suekia ; Masayuki Ueharab ; Mitsutoshi Suganoa ; Takeshi Ueharaa ; Takayuki Hondaa
- 关键词：APL ; acute promyelocytic leukemia ; AML ; acute myelogenous leukemia ; ATRA ; all-trans retinoic acid ; AsO3 ; arsenic trioxide ; droplet-RT-PCR ; droplet-reverse transcription-polymerase chain reaction ; FISH ; fluorescence in situ hybridization ; IQ ; instant quality ; RT-qPCR ; quantitative RT-PCR ; PB ; peripheral blood ; BM ; bone marrow
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：30 January 2016
- 年：2016
- 卷：453
- 期：Complete
- 页码：38-41
- 全文大小：384 K
文摘
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The droplet-RT-PCR can detect the PML-RARA gene within 26 min.
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The IQ-FISH yielded clear signals after 60 min of hybridization.
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The IQ-FISH showed same positive signal ratios as conventional FISH.
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We developed a rapid assay system for the PML-RARA fusion gene within 4 h.