Evaluation of saliva as diagnostic materials for influenza virus infection by PCR-based assays
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- 作者：Akane Suekia ; Kazuyuki Matsudaa ; kmatsuda@shinshu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Akemi Yamaguchib ; Masayuki Ueharab ; Mitsutoshi Suganoa ; Takeshi Ueharaa ; Takayuki Hondaa
- 关键词：Influenza virus ; Saliva ; Droplet-RT-PCR ; Rapid detection ; One-step real-time RT-PCR
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：30 January 2016
- 年：2016
- 卷：453
- 期：Complete
- 页码：71-74
- 全文大小：559 K
文摘
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High degree of result concordance was obtained from nasopharyngeal swabs and saliva.
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The droplet-RT-PCR assay could amplify influenza gene in the saliva within 14 min.
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Saliva contained influenza gene more than 1 × 102 copies/μl of influenza gene.
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About 50% of saliva was positive within 24 h after the onset of symptoms.