Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations
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- 作者：Sean C. Taylora ; sean_taylor@bio-rad.com" class="auth_mail" title="E-mail the corresponding author ; Julie Carbonneaub ; Dawne N. Sheltonc ; Guy Boivinb
- 关键词：Droplet Digital PCR ; Influenza virus ; Experimental optimization ; RT-qPCR ; ddPCR
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：224
- 期：Complete
- 页码：58-66
- 全文大小：2591 K
文摘
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Critical experimental optimization steps for Droplet Digital PCR (ddPCR).
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Hallmarks of a well-designed ddPCR assay.
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Direct comparison of multiplexed ddPCR and RT-qPCR under optimized conditions.
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Application of ddPCR for virus detection in clinical samples and comparison to RT-qPCR.