Performance of a real-time PCR-based approach and droplet digital PCR in detecting human parechovirus type 3 RNA
详细信息 查看全文
- 作者：Yuta Aizawaa ; Akihide Koyamab ; Tomohiko Ishiharab ; Osamu Onoderab ; Akihiko Saitoh ; M.D. ; Ph.D.a ; asaitoh@med.niigata-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Digital PCR ; Human parechovirus type 3 ; Real-time PCR ; Cerebrospinal fluid ; Neonates ; Infants
- 刊名：Journal of Clinical Virology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：27-31
- 全文大小：483 K
文摘
- •
Real-time RT-PCR detection limits for HPeV3 have not been fully evaluated.
- •
RT-ddPCR is the novel technique with precise quantitation of low-copy target genes.
- •
Two-step RT-ddPCR was less variable and more specific than one-step RT-ddPCR.
- •
Two-step RT-ddPCR detected HPeV3 RNA in real-time RT-PCR-negative CSF samples.