Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol
Cellulose membranes are a suitable matrix for steric exclusion chromatography. Virus particles were eluted in Tris-HCl and PBS buffers with >95% recovery. Maximum depletion of host cell DNA and protein was 99.7% and 92.4%, respectively. Operation is single-use and fully scalable to industrial manufacture. The DBC5 of the hemagglutinin antigen was 3.4 μgHA cm−2 (906 μgHA mL−1).