Antiviral effect of PmRab7 knock-down on inhibition of Laem-Singh virus replication in black tiger shrimp

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• 作者：Chalermporn Ongvarrasopone ; Ekapol Chomchay ; Sakol Panyim
• 关键词：Rab7 ; Penaeus monodon ; RNAi ; Double-stranded RNA
• 刊名：Antiviral Research
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：88
• 期：1
• 页码：116-118
• 全文大小：312 K

文摘

PmRab7 is a Penaeus monodon small GTPase protein possibly involved in replication of several shrimp viruses. In this study RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PmRab7 gene (dsRNA-PmRab7) was employed to silence the expression of PmRab7 to investigate the inhibitory effect on Laem-Singh virus (LSNV) replication. Injection of dsRNA-PmRab7 24 h before challenge with the virus resulted in a drastic decrease of PmRab7 mRNA and complete inhibition of LSNV replication at 3 days post-challenge. In a therapeutic mode, shrimp injected with dsRNA-PmRab7 1 day but not at 3 or 5 days post-LSNV challenge resulted in inhibition of LSNV replication. These results pave the way to use dsRNA-PmRab7 to prevent or cure LSNV infection in shrimp.