Forensic application of phylogenetic analyses - Exploration of suspected HIV-1 transmission case

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• 作者：Marina Siljic^a ; ¹ ; Dubravka Salemovic^b ; ¹ ; Valentina Cirkovic^a ; Ivana Pesic-Pavlovic^c ; Jovan Ranin^a ; ^b ; Marija Todorovic^a ; Slobodan Nikolic^d ; Djordje Jevtovic^a ; ^b ; Maja Stanojevic^a ; ^{mstanojevic@med.bg.ac.rs}
• 关键词：Phylogenetic analyses ; Forensic science ; Human immunodeficiency virus ; Transmission direction ; Transmission cluster
• 刊名：Forensic Science International: Genetics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：27
• 期：Complete
• 页码：100-105
• 全文大小：1028 K
• 卷排序：27

文摘

We present phylogenetic exploration, based on pol and env sequences, of suspected HIV transmission. End-point limiting-dilution PCR (EPLD-PCR) assay was performed to assess HIV quasispecies. Viral sequences from the query subjects formed strongly supported transmission cluster. Tree topology with paraphyletic relationship implies direction of transmission. Further study of the known and suspected transmission cases needed.