Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels
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- 作者：M. Ryan Smith<sup>asup><sup> ; sup><sup>bsup> ; Praveen K. Vayalil<sup>asup><sup> ; sup><sup>bsup> ; Fen Zhou<sup>asup><sup> ; sup><sup>bsup> ; Gloria A. Benavides<sup>asup><sup> ; sup><sup>bsup> ; Reena R. Beggs<sup>asup> ; Hafez Golzarian<sup>csup> ; Bhavitavya Nijampatnam<sup>csup> ; Patsy G. Oliver<sup>dsup> ; Robin A.J. Smith<sup>esup> ; Michael P. Murphy<sup>fsup> ; Sadanandan E. Velu<sup>csup> ; Aimee Landar<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>landar@uab.edu" class="auth_mail" title="E-mail the corresponding authorsup>
- 关键词：ACO ; aconitase ; BTPP ; butyl triphenylphosphonium ; DMEM/F12 ; Dulbecco's modified Eagle's medium/F12 ; ECAR ; extracellular acidification rate ; EtOH ; ethanol ; FBS ; fetal bovine serum ; FCCP ; carbonyl cyanide p-trifluoromethoxyphenylhydrazone ; GAC ; glutaminase C ; GAM ; glutaminase isoform 3 ; IBTP ; Iodobutyl triphenylphosphonium ; KGA ; kidney-type glutaminase ; MB231 ; MDA-MB231 ; MitoE ; mitochondria-targeted α-tocopherol ; MitoQ ; mitochondria-targeted ubiquinol ; MRM ; multiple reaction monitoring ; NEM ; N-eth
- 刊名：Redox Biology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：136-148
- 全文大小：3049 K
文摘
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IBTP dependent thiol modification decreases bioenergetics in MB231 and MCF-10A cells.
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IBTP treatment decreases ATP and other adenonucleotides after 1 to 6 days.
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IBTP treatment does not result in overt cellular toxicity.
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IBTP treatment decreases levels of bioenergetically-linked metabolites.
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IBTP treatment decreases aconitase activity and glutaminase protein levels.