Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells

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• 作者：Ilaria Trizio^a ; Eloisa Sardella^b ; Edda Francioso^c ; Giorgio Dilecce^b ; Vito Rizzi^a ; Pinalysa Cosma^a ; Michael Schmidt^d ; Mareike Hä ; nsch^d ; Thomas von Woedtke^d ; Pietro Favia^a ; ^b ; Roberto Gristina^b ; ^{roberto.gristina@cnr.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dielectric Barrier Discharge ; Eukaryotic cells ; Hydrogen peroxide ; Nitrite ; Nitrate
• 刊名：Clinical Plasma Medicine
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：3
• 期：2
• 页码：62-71
• 全文大小：2744 K

文摘

Atmospheric pressure (AP) Dielectric Barrier Discharges (DBD) generated in air were performed on water, Phosphate Buffer Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) in order to evaluate their potential to generate hydrogen peroxide (H₂O₂), nitrite (${\mathrm{NO}}_2^{-}$) and nitrate (${\mathrm{NO}}_3^{-}$) species. Differences in cell growth and morphology were found in two different types of eukaryotic cells, immortal and stem cells, incubated for 1 h in plasma treated DMEM, revealing a selectivity of the treatment.