Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions
详细信息 查看全文
- 作者：Alaina C. Schlinker ; Mark T. Duncan1 ; Teresa A. DeLuca1 ; David C. Whitehead ; William M. Miller ; wmmiller@northwestern.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Culture-derived platelets ; Animal cell culture ; Tissue culture ; Cell adhesion ; Biomedical ; Physiology
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：111
- 期：Complete
- 页码：24-33
- 全文大小：3589 K
文摘
- •
We cultured MKs on tissue-culture treated (TC) and Ultra Low Attachment (ULA) surfaces.
- •
We examined changes in MK polyploidization and proplatelet formation (PPF).
- •
CHRF cells displayed increased polyploidization and arrested PPF on a ULA surface.
- •
ULA culture did not affect primary MK polyploidization, but decreased PPF.
- •
Primary MKs and CHRF cells showed rapid PPF after transfer from a ULA to a TC surface.