Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat

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• 作者：Christiane Takayama¹ ; Felipe Meira de-Faria² ; Ana Cristina Alves de Almeida¹ ; Ricardo José ; Dunder² ; Luis Paulo Manzo² ; Eduardo Augusto Rabelo Socca² ; Leonia Maria Batista³ ; Marcos José ; Salvador⁴ ; Alba Regina Monteiro Souza-Brito¹ ; ² ; Anderson Luiz-Ferreira⁵ ; ^{luiz_ferreira@ufg.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Rosmarinus officinalis L. ; Antioxidant activity ; Gastric ulcer ; Antioxidant enzymes
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：6
• 期：8
• 页码：677-681
• 全文大小：353 K

文摘

To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis (R. officinalis) in ethanol-induced gastric ulcer model in vivo.

Methods

The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.

Results

Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of (140.2 ± 37.2) mm². In animals pretreated with essential oil of R. officinalis (50 mg/kg, p.o.), a significant inhibition of mucosal injury of (21.2 ± 7.1) mm² (84% inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes (superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition, lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole (28.5%), camphor (27.7%) and alpha-pinene (21.3%).

Conclusions

We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.