Solution properties of capsular polysaccharides from Streptococcus pneumoniae

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• 作者：Stephen E. Harding ; Ali Saber Abdelhameed ; Gordon A. Morris ; Gary Adams ; Olivier Laloux ; Louis Cerny ; Benjamin Bonnier ; Pierre Duvivier ; Karel Conrath ; Christophe Lenfant
• 关键词：Bacterial ; Vaccines ; Hydrodynamics ; Size distribution ; Conformation
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 出版时间：1 September, 2012
• 年：2012
• 卷：90
• 期：1
• 页码：237-242
• 全文大小：534 K

文摘

Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L_p), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100 ¡Á 10³ to 1300 ¡Á 10³ g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ¡«4 to 9 nm.