Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

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• 作者：Wen CHENG^a ; Jin-shan GAO^b ; Xing-xing FENG^b ; Qun SHAO^a ; Su-xin YANG^b ; Xian-zhong FENG^a ; ^b ; ^{fengxianzhong@iga.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：soybean ; dwarf mutant ; mapping ; BR ; GA
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：15
• 期：10
• 页码：2228-2236
• 全文大小：1946 K

文摘

Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M₂ population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.