We report on a new late Miocene elasmobranch assemblage from Cerro Colorado (Pisco Fm). This assemblage includes at least 21 species attributed to 9 families and 5 orders. 7 taxa are recorded for the first time in the Pisco Fm and 3 in the fossil record of Peru. Cerro Colorado was exploited as a nursery area. Ecological and trophic dynamics have been recognized over the stratigraphic succession.