Analysis of two Schistosoma mansoni uridine phosphorylases isoforms suggests the emergence of a protein with a non-canonical function
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- 作者：Antô ; nio Marinho da Silva Netoa ; Juliana Roberta Torini de Souzaa ; Larissa Romanelloa ; Alexandre Cassagob ; Vitor Hugo Balasco Serrã ; oa ; Ricardo DeMarcoa ; José ; Brandã ; o-Netoc ; Richard Charles Garratta ; Humberto D'Muniz Pereiraa ; hmuniz@ifsc.usp.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Schistosoma mansoni ; Uridine phosphorylase ; X-ray crystallography ; Isoforms ; Pyrimidine metabolism
- 刊名：Biochimie
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：12-22
- 全文大小：3093 K
文摘
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Structures of two uridine phosphorylases isoforms from S. mansoni in APO form and bound to different ligands is presented.
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The kinetic analyses showed that SmUPa is a regular uridine phosphorylase but SmUPb presented no detectable activity.
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Despite 92% sequence identity, the main difference resides at the active site, indicating different functions.