Separation of steroid isomers by ion mobility mass spectrometry

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• 作者：Linda Ahonen ; Ma¨ªra Fasciotti ; Gustav Boije af Genn?s ; Tapio Kotiaho ; Romeu J. Daroda ; Marcos Eberlin ; Risto Kostiainen
• 关键词：Ion mobility mass spectrometry ; Steroids ; Steroid isomers ; Derivatization ; Collision cross section
• 刊名：Journal of Chromatography A
• 出版年：2013
• 出版时间：4 October, 2013
• 年：2013
• 卷：1310
• 期：Complete
• 页码：133-137
• 全文大小：481 K

文摘

Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (R_p ? p = 0.77-1.08).