Microorganisms in cryopreserved semen and culture media used in the in?vitro production (IVP) of bovine embryos identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)

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• 作者：D¨¢vila Zampieri ; Vanessa G. Santos ; Patr¨ªcia A.C. Braga ; Christina R. Ferreira ; Daniela Ballottin ; Ljubica Tasic ; Andr¨¦a C. Basso ; Bruno V. Sanches ; Jos¨¦ H.F. Pontes ; B¨¢rbara Pereira da Silva ; Fabiana Fantinatti Garboggini ; Marcos N. Eberlin ; Aless ; ra Tata
• 关键词：Matrix-assisted laser desorption ionization mass spectrometry ; Microorganisms ; Bacteria ; Semen ; Culture media ; In vitro production
• 刊名：Theriogenology
• 出版年：2013
• 出版时间：1 September, 2013
• 年：2013
• 卷：80
• 期：4
• 页码：337-345
• 全文大小：891 K

文摘

Commercial cattle breeders produce their own herd offspring for the dairy and beef market using artificial insemination. The procedure involves sanitary risks associated with the collection and commercialization of the germplasm, and the in?vitro production and transfer of the bovine embryos must be monitored by strict health surveillance. To avoid the spreading of infectious diseases, one must rely on using controlled and monitored germplasm, media, and reagents that are guaranteed free of pathogens. In this article, we investigated the use of a new mass spectrometric approach for fast and accurate identification of bacteria and fungi in bovine semen and in culture media employed in the embryo in?vitro production process. The microorganisms isolated from samples obtained in a commercial bovine embryo IVP setting were identified in a few minutes by their conserved peptide/protein profile, obtained applying matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), matched against a commercial database. The successful microorganisms MS identification has been confirmed by DNA amplification and sequencing. Therefore, the MS technique seems to offer a powerful tool for rapid and accurate microorganism identification in semen and culture media samples.