Elevated IL-35 in bone marrow of the patients with acute myeloid leukemia

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• 作者：Jia Wang^a ; Qianshan Tao^a ; ^b ; Huiping Wang^a ; ^b ; Zhitao Wang^a ; Fan Wu^a ; ^b ; Ying Pan^a ; ^b ; Lili Tao^a ; ^b ; Shudao Xiong^a ; ^b ; Yiping Wang^c ; Zhimin Zhai^a ; ^b ; ^{zzzm889@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AML ; acute myeloid leukemia ; HSCT ; hematopoietic stem cell transplantation ; Tregs ; regulatory T cells ; EBI3 ; Epstein&ndash ; Barr virus induced gene 3 ; ND ; newly diagnosed ; CR ; complete remission ; BMMCs ; bone marrow mononuclear cells ; iTr35 cells ; IL-35-producing induced regulatory T-cells ; aGVHD ; acute-graft-versus-host disease ; allo-HSCT ; allogeneic hematopoietic stem cell transplantation
• 刊名：Human Immunology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：76
• 期：9
• 页码：681-686
• 全文大小：869 K

文摘

Acute myeloid leukemia (AML) is the most common hematological malignancy in adults, but the etiology of it remains poorly understood. IL-35 is a recently described cytokine composed of an IL-12 subunit p35 and an IL-27 subunit Epstein–Barr virus induced gene 3 (EBI3), and has an immunosuppressive effect on inflammation through induction of regulatory T cells (Tregs) and suppression of Th1 and Th17. Recently, we have illustrated that concentrations of IL-35 in peripheral blood are up-regulated in newly diagnosed (ND) AML patients. However, whether IL-35 in bone marrow is increased in AML patients is not clear. In this study, we examined IL-35 in bone marrow by various methods including RT-PCR, ELISA, FCM and IHC, and found that IL-35 levels are also increased significantly in bone marrow of adult AML patients. Furthermore, we investigated that concentrations of bone marrow IL-35 in ND group were higher than that in complete remission (CR) group and control group, but there was no significant difference compared to that in relapse group. In conclusion, IL-35 was elevated in bone marrow of adult AML patients and this increase was correlated with the clinical stages of malignancy, suggesting that IL-35 is involved in pathogenesis of AML.