SLPI prevents cytokine release in mite protease-exposed conjunctival epithelial cells

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• 作者：Takahiko Seto ; Toshiro Takai ; Nobuyuki Ebihara ; Hiroyuki Matsuoka ; Xiao-Ling Wang ; Akira Ishii ; Hideoki Ogawa ; Akira Murakami ; Ko Okumura
• 关键词：Ocular conjunctival epithelial cells ; House dust mite ; Serine protease ; Proinflammatory cytokine response ; Innate antiproteases ; Secretory leukocyte protease inhibitor ; α ; 1-antitrypsin ; Allergic conjunctivitis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：13 February 2009
• 年：2009
• 卷：379
• 期：3
• 页码：681-685
• 全文大小：219 K

文摘

House dust mites are a major source of allergens associated with allergic diseases including allergic conjunctivitis. Here, we demonstrate that mite-derived serine protease activity induces the release of cytokines from human ocular conjunctival epithelial cells in vitro and innate antiproteases, secretory leukocyte protease inhibitor (SLPI) and α1-antitrypsin, can inhibit the response. An extract prepared from a whole-mite culture induced the release of IL-6 and IL-8 and upregulated their gene expression in the human conjunctival epithelial cell line Chang, responses which were inhibited not only by a synthetic serine protease-specific inhibitor, AEBSF, but also by SLPI and α1-antitrypsin at a physiologically relevant concentration. The findings suggest a homeostatic role for SLPI and α1-antitrypsin against the proteases contained in allergen sources in the ocular conjunctiva and that exposure to house dust particles containing mite-derived serine protease activity could be involved in the initiation of sensitization through the ocular conjunctival epithelium and/or exacerbation of allergic conjunctivitis.