Biochemical characterization and immobilization of Erwinia carotovora l-asparaginase in a microplate for high-throughput biosensing of l-asparagine
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- 作者：Nikolaos E. Labroua ; Lambrou@aua.gr" class="auth_mail" title="E-mail the corresponding author ; Magdy Mohamed Muharramb ; c
- 关键词：BSA ; bovine serum albumin ; EcaL-ASNase ; l-asparaginase from Erwinia carotovora ; EcA ; l-asparaginase from Escherichia coli ; ErA ; l-asparaginase from Erwinia chrysantemi ; IPTG ; isopropylβ-d-1-thiogalactopyranoside ; l-Asn ; l-asparagine ; l-Asp ; aspartic acid ; l-Gln ; l-glutamine ; PAGE ; polyacryamide gel electrophoresis ; SDS ; sodium dodecyl sulfate ; Suc ; succinic acid
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：92
- 期：Complete
- 页码：86-93
- 全文大小：2916 K
文摘
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l-ASNase from E. carotovora was cloned, expressed and characterized.
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The enzyme was used to assemble a microplate-based biosensor.
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The biosensor was explored for the determination of l-Asn in biological samples.
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The biosensor displayed high specificity and good stability.