Pyrrolizidine alkaloids in two endemic capeverdian Echium species

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• 作者：Jos¨¦ Carlos B. Carvalho ; Henrique dos Santos Almeida ; Jonathas Felipe Revoredo Lobo ; Jos¨¦ Luiz Pinto Ferreira ; Adriana Passos Oliveira ; Le ; ro Rocha
• 关键词：Echium stenosiphon ; Echium hypertropicum ; Boraginaceae ; Pyrrolizidine alkaloids ; Cape Verde
• 刊名：Biochemical Systematics and Ecology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：50
• 期：Complete
• 页码：1-6
• 全文大小：264 K

文摘

Echium hypertropicum Webb and Echium stenosiphon Webb subsp. stenosiphon are capeverdian shrubs used in folk medicine for the treatment of cough and gastrointestinal diseases. Acid-base extraction was used to obtain alkaloid-rich fractions from both species. GC-MS and ESI-MS/MS analysis indicated the presence of pyrrolizidine alkaloids (PAs) and purified substances were also analyzed by 1D and 2D NMR experiments. A total of ten alkaloids were detected, eight of which were identified by comparing their molecular masses and mass fragmentation patterns with the NIST database and data in the literature. The hepatotoxic diesters echimidine and 7-(2-methylbutyryl)-9-echimidinylretronecine were identified in both species. Echimidine was the major component in the diethyl ether fraction from leaves of E. hypertropicum, whereas the 7-(2-methylbutyryl)-9-echimidinylretronecine was the major component in the dichloromethane fraction from leaves of E. stenosiphon. To our knowledge, this is the first report on the occurrence of pyrrolizidine alkaloids in capeverdian species of Echium. This study on Echium led to the identification of constituent pyrrolizidine alkaloids, serving to assist taxonomists with the complex taxonomy of this genus.