A novel method for assaying human regulatory T cell direct suppression of B cell effector function

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• 作者：Elizabeth Weingartner ^{eweingartner@umaryland.edu} ; Jean-Paul Courneya ^{jpcourneya@hshsl.umaryland.edu} ; Achsah Keegan¹ ; ^{akeegan@som.umaryland.edu} ; Amit Golding ; ^{agolding@medicine.umaryland.edu}
• 关键词：Treg ; Autoimmunity ; B cell ; Antibody ; Suppression assay ; Human
• 刊名：Journal of Immunological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：441
• 期：Complete
• 页码：1-7
• 全文大小：995 K
• 卷排序：441

文摘

We have established a highly reproducible and reliable protocol for testing human regulatory T cell function in suppressing IgM production from an immature human B cell line. The autoreactive Ramos B cell line provides a stable reporter of B cell effector function that can be tested by a straight-forward IgM ELISA. Tregs from healthy volunteers display a range of ability for suppressing baseline IgM production in a contact- and death-independent manner. Having established the normal range for human Treg direct suppression of B cell effector function, it will now be possible to efficiently test Tregs from various autoimmune conditions in which B cell hyperactivity and secretion of auto-antibodies are a hallmark of disease.