Importin α is an essential nuclear import carrier adaptor required for proper sexual and asexual development and secondary metabolism in Aspergillus nidulans

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• 作者：Lidia Araú ; jo-Bazá ; n ; Sourabh Dhingra ; John Chu ; Javier Ferná ; ndez-Martí ; nez ; Ana M. Calvo ; Eduardo A. Espeso
• 关键词：Aspergillus nidulans ; Nuclear transport ; Karyopherin ; Conditional mutation ; Sexual development ; Asexual development ; Secondary metabolism
• 刊名：Fungal Genetics and Biology
• 出版年：2009
• 出版时间：June-July 2009
• 年：2009
• 卷：46
• 期：6-7
• 页码：506-515
• 全文大小：1028 K

文摘

In eukaryotes, the principal nuclear import pathway is driven by the importin α/β1 heterodimer. KapA, the Aspergillus nidulans importin α, is an essential protein. We generated a conditional allele, kapA31, mimicking the srp1-31 allele in Saccharomyces cerevisiae. KapA31 carries a Ser111Phe amino acid substitution which, at the restrictive temperature of 42 °C, reduces nuclear import of cargos containing classical nuclear-localization-sequences, cNLS. Using kapA31, we have demonstrated the role of the importin α in the nuclear accumulation of the light-dependent developmental regulator VeA. KapA have additional tasks in the cell, as reported for other members of the importin α family. KapA participates at different regulatory stages of asexual and sexual development, being required for the completion of both reproductive cycles with the formation of conidiospores and ascospores, respectively. Finally, KapA also mediates in different pathways of secondary metabolism having a dual role: positively for penicillin production and negatively for mycotoxin biosynthesis.