• 作者：Wenjie Yin^a ; ^b ; Laurent Gorvel^c ; Sandra Zurawski^a ; Dapeng Li^a ; Ling Ni^a ; Dorothé ; e Duluc^a ; Katherine Upchurch^a ; ^b ; JongRok Kim^a ; Chao Gu^a ; ^b ; Richard Ouedraogo^a ; Zhiqing Wang^a ; Yaming Xue^a ; HyeMee Joo^a ; ^b ; Jean-Pierre Gorvel^d ; Gerard Zurawski^a ; ^b ; SangKon Oh^a ; ^b ; ^{sangkono@baylorhealth.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ANOVA ; analysis of variance ; AP ; alkaline phosphatase ; APC ; antigen-presenting cells ; CD ; cluster of differentiation ; CFSE ; carboxyfluorescein succinimidyl ester ; Coh ; cohesin ; CTL ; cytotoxic T lymphocyte ; DC ; dendritic cell ; EEA1 ; early endosome antigen 1 ; ELISA ; enzyme-linked immunosorbent assay ; ELISpot ; enzyme-linked immunospot ; Doc ; dockerin ; Flu.M1 ; influenza virus matrix protein 1 ; GM-CSF ; granulocyte-macrophage colony-stimulating factor ; HA1 ; hemagglutinin subunit 1 ; hCD40Tg ; human CD40
• 刊名：EBioMedicine
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：5
• 期：Complete
• 页码：46-58
• 全文大小：1924 K

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Antigen delivery to DCs via CD40 is more efficient than through nine other receptors at eliciting CD8 T⁺ cell response.

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Antigen delivery via lectins (e.g., LOX-1 and Dectin-1) is more efficient than CD40 at eliciting CD4⁺ T cell responses.

The success of an immunotherapeutic vaccine for cancer is largely dependent on its ability to evoke potent cellular immunity. Although targeting antigens to dendritic cells (DCs) has been known to be an efficient strategy to evoke cellular immunity, which targeted receptors yield the optimal cellular immunity remained elusive. We report that targeting CD40, compared to 9 other DC receptors, results in the greatest levels of CD8⁺ cytotoxic T cell responses, while targeting lectins results in enhanced CD4⁺ helper T cell responses. The findings of this study will assist us in the rational design of immunotherapeutic vaccines against cancers.