Engineering the transposition-based baculovirus expression vector system for higher efficiency protein production from insect cells
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- 作者：Jennifer L. Mehalko ; Dominic Esposito ; dom.esposito@nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：AcMNPV ; Autographa californica multiple nucleopolyhedrovirus ; GST ; glutathione-S-transferase ; MBP ; maltose-binding protein ; CCP ; combinatorial cloning platform ; cfu ; colony forming units
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 November 2016
- 年：2016
- 卷：238
- 期：Complete
- 页码：1-8
- 全文大小：1124 K
文摘
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A modified set of vectors and strains improves baculovirus production.
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Transposition frequency can be enhanced by vector and strain engineering.
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Optimized protocols permit higher throughput baculovirus production.