Altered primordial germ cell migration in the absence of transforming growth factor β signaling via ALK5

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• 作者：Susana M. Chuva de Sousa Lopes ; Sander van den Driesche ; Rita L.C. Carvalho ; Jonas Larsson ; Bart Eggen ; M. Azim Surani and Christine L. Mummery
• 关键词：Primordial germ cells ; Mouse embryo ; TGFβ ; Migration ; Collagen ; Hindgut
• 刊名：Developmental Biology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：284
• 期：1
• 页码：194-203
• 全文大小：627 K

文摘

Transforming growth factor β (TGFβ) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGFβ signaling via the type I receptor ALK5. TGFβ in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E)10. Unexpectedly, the absence of TGFβ signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGFβ signaling in migration of PGCs in vivo distinct from that described previously.