The 3D hierarchical porous Co3O4 film was prepared by employing eggshell membrane as bio-templates.
The 3D hierarchical film is more effective in facilitating reactants diffusion and the electron transfer.
The Co3O4-based sensor was applied for direct electrochemical non-enzymatic detection of glucose.
It shows high sensitivity (366.03 μAmM− 1 cm− 2), fast response (3 s), excellent stability and anti-interference performance.