Early growth response 2 (Egr2) plays opposing roles in committing C3H10T1/2 stem cells to adipocytes and smooth muscle-like cells

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• 作者：Shan-Shan Wang ; Hai-Yan Huang ; Su-Zhen Chen ; Xi Li ; Yang Liu ; Wen-Ting Zhang ; Qi-Qun Tang
• 关键词：MSC ; mesenchymal stem cell ; SMC ; smooth muscle cell ; BMP4 ; bone morphogenetic protein 4 ; Egr2 ; early growth response 2 ; Egr1 ; early growth response 1 ; Tgf¦Â1 ; transforming growth factor ¦Â1 ; ¦Á-SMA ; actin alpha 2 smooth muscle aorta ; Srf ; serum response f
• 刊名：The International Journal of Biochemistry and Cell Biology
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：45
• 期：8
• 页码：1825-1832
• 全文大小：1790 K

文摘

Early growth response 2 (Egr2) is a zinc-finger transcription factor that acts as an important modulator of a variety of physiological processes, such as cell differentiation, proliferation and apoptosis. Here we showed that Egr2 was downregulated by bone morphogenetic protein (BMP) signaling pathways during the commitment of C3H10T1/2 stem cells to adipocyte lineage. Overexpression of Egr2 completely prevented BMP4-induced adipocyte lineage commitment of C3H10T1/2 stem cells, while simultaneously stimulating early smooth muscle-like differentiation. We also demonstrated that Egr2-induced early smooth muscle-like differentiation is transforming growth factor ¦Â1-independent. Egr2 can activate the transcription of early smooth muscle cell specific genes smooth muscle protein 22¦Á and calponin 1. Together, the results indicated a novel role for Egr2 in repressing adipocyte lineage commitment and promoting early smooth muscle-like cell differentiation.