Molecular Evolution of the Substrate Specificity of Chloroplastic Aldolases/Rubisco Lysine Methyltransferases in Plants

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• 作者：Sheng Ma¹ ; ² ; ³ ; ⁴ ; Jacqueline Martin-Laffon¹ ; ² ; ³ ; ⁴ ; Morgane Mininno¹ ; ² ; ³ ; ⁴ ; Océ ; ane Gigarel¹ ; ² ; ³ ; ⁴ ; Sabine Brugi&egrave ; re⁵ ; ⁶ ; ⁷ ; Olivier Bastien¹ ; ² ; ³ ; ⁴ ; Marianne Tardif⁵ ; ⁶ ; ⁷ ; Sté ; phane Ravanel¹ ; ² ; ³ ; ⁴ ; Claude Alban¹ ; ² ; ³ ; ⁴ ; ^{claude.alban@cea.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：SET-domain protein methyltransferase ; site-directed mutagenesis ; chimeric enzyme ; methylation ; molecular evolution ; green lineage
• 刊名：Molecular Plant
• 出版年：2016
• 出版时间：4 April 2016
• 年：2016
• 卷：9
• 期：4
• 页码：569-581
• 全文大小：2814 K

文摘

Rubisco and fructose-1,6-bisphosphate aldolases (FBAs) are involved in CO₂ fixation in chloroplasts. Both enzymes are trimethylated at a specific lysine residue by the chloroplastic protein methyltransferase LSMT. Genes coding LSMT are present in all plant genomes but the methylation status of the substrates varies in a species-specific manner. For example, chloroplastic FBAs are naturally trimethylated in both Pisum sativum and Arabidopsis thaliana, whereas the Rubisco large subunit is trimethylated only in the former species. The in vivo methylation status of aldolases and Rubisco matches the catalytic properties of AtLSMT and PsLSMT, which are able to trimethylate FBAs or FBAs and Rubisco, respectively. Here, we created chimera and site-directed mutants of monofunctional AtLSMT and bifunctional PsLSMT to identify the molecular determinants responsible for substrate specificity. Our results indicate that the His-Ala/Pro-Trp triad located in the central part of LSMT enzymes is the key motif to confer the capacity to trimethylate Rubisco. Two of the critical residues are located on a surface loop outside the methyltransferase catalytic site. We observed a strict correlation between the presence of the triad motif and the in vivo methylation status of Rubisco. The distribution of the motif into a phylogenetic tree further suggests that the ancestral function of LSMT was FBA trimethylation. In a recent event during higher plant evolution, this function evolved in ancestors of Fabaceae, Cucurbitaceae, and Rosaceae to include Rubisco as an additional substrate to the archetypal enzyme. Our study provides insight into mechanisms by which SET-domain protein methyltransferases evolve new substrate specificity.