The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants

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• 作者：Astrid Gadeyne ; Clara S谩nchez-Rodr铆guez ; Steffen Vanneste ; Simone Di聽Rubbo ; Henrik Zauber ; Kevin Vanneste ; Jelle Van聽Leene ; Nancy De聽Winne ; Dominique Eeckhout ; Geert Persiau ; Eveline Van聽De聽Slijke ; Bernard Cannoot ; Leen Vercruysse ; Jonathan聽R. Mayers ; Maciek Adamowski ; Urszula Kania ; Matthias Ehrlich ; Alois Schweighofer ; Tijs Ketelaar ; Steven Maere ; Sebastian聽Y. Bednarek ; et al.
• 刊名：Cell
• 出版年：13 February, 2014
• 年：2014
• 卷：156
• 期：4
• 页码：691-704
• 全文大小：4190 K

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Summary

Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex聽is an early endocytic module representing a聽unique聽evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.