Flow cytometric characterization of microglia in the offspring of PolyI:C treated mice
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- 作者：Marie Pierre Manitza ; marie-pierre.manitz@rub.de" class="auth_mail" title="E-mail the corresponding author ; Jennifer Plü ; mpera ; Seray Demirb ; 1 ; seray.demir@rub.de" class="auth_mail" title="E-mail the corresponding author ; Maike Ahrensc ; 2 ; maike.ahrens@rub.de" class="auth_mail" title="E-mail the corresponding author ; Manuela Eß ; lingera ; 3 ; manuela.esslinger@rub.de" class="auth_mail" title="E-mail the corresponding author ; Simone Wachholza ; 4 ; simone.wachholz@rub.de" class="auth_mail" title="E-mail the corresponding author ; Martin Eisenacherc ; 5 ; martin.eisenacher@rub.de" class="auth_mail" title="E-mail the corresponding author ; Georg Juckela ; 6 ; georg.juckel@wkp-lwl.org" class="auth_mail" title="E-mail the corresponding author ; Astrid Friebea ; 7 ; astrid.friebe@rub.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Microglia ; Flow cytometry ; Iba1 ; CD11b ; CD45 ; Morphology ; Cellular activation ; PolyI ; C
- 刊名：Brain Research
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1636
- 期：Complete
- 页码：172-182
- 全文大小：1979 K
文摘
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Iba1 is a reliable antibody to analyze its expression via flow cytrometry.
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Iba1 expression in the PolyI:C model indicates changes in microglial activation.
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Differences in expression of CD11b, CD45 and Iba1 are age and gender-specific.
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PolyI:C leads to age and gender specific alterations in CD11b and CD45 expression.