Guided growth of auditory neurons: Bioactive particles towards gapless neural - electrode interface

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• 作者：Hao Li^a ; Fredrik Edin^a ; Hisamitsu Hayashi^b ; Olafur Gudjonsson^c ; Niklas Danckwardt-Lillieströ ; m^a ; Hå ; kan Engqvist^d ; Helge Rask-Andersen^a ; ^{Helge.Rask-andersen@surgsci.uu.se} ; Wei Xia^d ; ^{Wei.xia@angstrom.uu.se}
• 关键词：Cochlear implant ; Neurotrophin ; 3D culture ; Bioactive spheres ; Calcium phosphate
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：122
• 期：Complete
• 页码：1-9
• 全文大小：2528 K
• 卷排序：122

文摘

Cochlear implant (CI) is a successful device to restore hearing. Despite continuous development, frequency discrimination is poor in CI users due to an anatomical gap between the auditory neurons and CI electrode causing current spread and unspecific neural stimulation. One strategy to close this anatomical gap is guiding the growth of neuron dendrites closer to CI electrodes through targeted slow release of neurotrophins. Biodegradable calcium phosphate hollow nanospheres (CPHSs) were produced and their capacity for uptake and release of neurotrophins investigated using 125I-conjugated glia cell line-derived neurotrophic factor (GDNF). The CPHSs were coated onto CI electrodes and loaded with neurotrophins. Axon guidance effect of slow-released neurotrophins from the CPHSs was studied in an in vitro 3D culture model. CPHS coating bound and released GDNF with an association rate constant 6.3 × 103 M−1s−1 and dissociation rate 2.6 × 10−5 s−1, respectively. Neurites from human vestibulocochlear ganglion explants found and established physical contact with the GDNF-loaded CPHS coating on the CI electrodes placed 0.7 mm away. Our results suggest that neurotrophin delivery through CPHS coating is a plausible way to close the anatomical gap between auditory neurons and electrodes. By overcoming this gap, selective neural activation and the fine hearing for CI users become possible.