Highly sensitive amperometric detection of cardiac troponin I using sandwich aptamers and screen-printed carbon electrodes

详细信息    查看全文

• 作者：Hunho Jo^a ; ^{jhhsst@postech.ac.kr} ; Jin Her^a ; ^{jinh0902@postech.ac.kr} ; Heehyun Lee^b ; ^{delighthyun@postech.ac.kr} ; Yoon-Bo Shim^c ; ^{ybshim@pusan.ac.kr} ; Changill Ban^a ; ^{ciban@postech.ac.kr}
• 关键词：Screen-printed carbon electrode ; Cardiac troponin I ; Aptamer ; Chronoamperometry ; Hydrazine ; Diagnosis
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：442-448
• 全文大小：821 K
• 卷排序：165

文摘

Screen-printed carbon electrode (SPCE)-based aptasensor for cTnI. Highly sensitive detection using a sandwich aptamer assay. Robust amperometric signals from catalytic reaction between hydrazine and H2O2. A low detection limit of 1 pM in a buffer as well as in a serum solution. Great selectivity toward only cTnI over other proteins.