ERGs were recorded with both electrode types in nine healthy Brown-Norway rats under scotopic conditions. For low intensity responses a Naka–Rushton function was fitted and the parameters Vmax, k and n were analyzed. The a-wave, b-wave and oscillatory potentials were analyzed for brighter flash intensities (1–60a0;scota0;cda0;s/m2). Repeatability was assessed for both electrode types in consecutive measurements.
The new electrode holder was faster in setting up than the CLE and showed lower standard deviations. No corneal alterations were observed. Slightly higher amplitudes were recorded in most of the measurements with the new electrode holder (except amplitudes induced by 60a0;cda0;s/m2). A Bland–Altman test showed good agreement between the DTL holder and the CLE (mean difference 35.2a0;μV (Holder-CLE)). Pearson's correlation coefficient for test–retest-reliability was ra0;=a0;0.783.
The DTL holder was superior in handling and caused far less corneal problems than the CLE and produced comparable or better electrophysiological results. The minimal production costs and the possibility of adapting the DTL holder to bigger eyes, such as for dogs or rabbits, offers with broader application prospects.