3D microfluidic origami electrochemiluminescence immunodevice for sensitive point-of-care testing of carcinoma antigen 125

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• 作者：Shaowei Wang^aAuthor Vitae ; Lei Ge^aAuthor Vitae ; Mei Yan^aAuthor Vitae ; Jinghua Yu^a ; ^{ujn.yujh@gmail.com}Author Vitae ; Xianrang Song^b ; ^{benben880208@163.com}Author Vitae ; Shenguang Ge^a ; ^cAuthor Vitae ; Jiadong Huang^aAuthor Vitae
• 关键词：Microfluidic origami device ; Screen-printed electrodes ; Electrochemiluminescence immunosensor ; Tumor markers ; Point-of-care testing
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：176
• 期：Complete
• 页码：1-8
• 全文大小：1264 K

文摘

In this paper, a 3D microfluidic origami device combined with electrochemiluminescence (ECL) immunosensor was introduced for sensitive point-of-care testing of carcinoma antigen 125 in clinical serum samples for the first time. This microfluidic origami device was fabricated by directly screen-printing carbon working, counter electrodes and Ag/AgCl reference electrode including their conductive pads on wax-patterned pure cellulose paper, and was activated by folding to form a 3D electrochemical cell. To construct a sensitivity-enhanced sandwich-type ECL immunosensor, gold nanoparticles (AuNPs) was synthesized to modify the working electrode to provide a good pathway of electron transfer and enhance the immobilized amount of capture antibody (McAb₁), and luminol functionalized gold nanoparticles (Lu-AuNPs) was synthesized to label signal antibody (McAb₂). With the aid of a facile device-holder, cyclic voltammetry was applied on microfluidic origami ECL immunodevice to trigger ECL. Under optimal conditions, this microfluidic origami ECL immunodevice provided a good linear response range from 0.01 to 100 U mL^?1 with a detection limit of 0.0074 U mL^?1. The proposed ECL immunodevice presented high sensitivity and stability, and could be applied in point-of-care testing of other tumor markers for remote regions and developing countries.