Parallel analysis and orthogonal identification of N-glycans with different capillary electrophoresis mechanisms

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• 作者：Hua-tao Feng^a ; ^b ; Min Su^a ; Farida Nur Rifai^a ; Pingjing Li^b ; Sam F.Y. Li^a ; ^b ; ^{chmlifys@nus.edu.sg}
• 关键词：Multiplexing ; CE ; Glycan ; Separation ; Glycoprotein ; LIF ; Oligosaccharides ; CZE ; MEKC
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：8 February 2017
• 年：2017
• 卷：953
• 期：Complete
• 页码：79-86
• 全文大小：1874 K
• 卷排序：953

文摘

Cross-validation of analytes in complex samples was done with different CE separation mechanisms. A simple strategy is used to confirm peak identification and extend capacity of CE separation. The method uses small amount of sample, simple instrument and single fluorescent labeling. Selection of mechanisms is based on orthogonalities of GU values of glycan standards. Micellar electrokinetic chromatography was suitable for analysis of small or highly sialylated glycans.