Induction of electrophile-responsive element (EpRE)-mediated gene expression by tomato extracts in vitro
详细信息 查看全文
- 作者：Linda Gijsbers ; Henri?tte D.L.M. van Eekelen ; Thuy H. Nguyen ; Laura H.J. de Haan ; Bart van der Burg ; Jac M.M.J.G. Aarts ; Ivonne M.C.M. Rietjens ; Arnaud G. Bovy
- 关键词：Tomato ; Solanum lycopersicum ; Extracts ; Electrophile-responsive element (EpRE) ; Phenolic compounds ; Isoprenoids
- 刊名：Food Chemistry
- 出版年：2012
- 出版时间：1 December, 2012
- 年：2012
- 卷：135
- 期：3
- 页码：1166-1172
- 全文大小：346 K
文摘
The market for food products with additional health benefits is increasing rapidly and tools for identification of bio-functional characteristics of food items are essential. To facilitate the detection of beneficial effects of tomato on gene expression, methods to prepare tomato extracts suitable to test in the EpRE LUX assay and other cell-based reporter gene assays for health-related bioactivity mechanisms, were developed. An isoprenoid-containing chloroform extract of tomato fruit and most individual isoprenoids did not induce electrophile-responsive element (EpRE)-mediated gene expression. A semi-polar extract of tomato fruits, enzymatically hydrolysed to remove the glycosyl residues from the phenolic ingredients was able to induce EpRE-mediated luciferase expression at both mRNA and protein level, which might be partly due to the presence of quercetin, kaempferol, naringenin and naringenin chalcone. It was concluded that induction of EpRE-regulated genes, such as detoxifying phase II and antioxidant enzymes, may contribute to the beneficial health effects of tomato.