In vitro studies of enzymatic properties of starch synthases and interactions between starch synthase I and starch branching enzymes from rice
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- 作者：Yasunori Nakamura ; nakayn@silver.plala.or.jp" class="auth_mail ; Satomi Aihara ; Naoko Crofts ; Takayuki Sawada ; Naoko Fujita
- 关键词：APTS ; 8-amino-1 ; 3 ; 6-pyrenetrisulfonic acid ; BE ; starch branching enzyme ; CD ; cluster dextrin ; DP ; degree of polymerization ; E. coli ; Escherichia coli ; FACE ; fluorophore-assisted carbohydrate capillary electrophoresis ; G1P ; glucose 1-phosphate ; G6 ; maltohexaose ; KpPUL ; Klebsiella pneumoniae pullulanase ; MOS ; malto-oligosaccharides ; PaISA ; Pseudomonas amyloderamosa isoamylase ; Pho1 ; plastidial phosphorylase ; SS ; starch synthase
- 刊名：Plant Science
- 出版年：July 2014
- 年：2014
- 卷：224
- 期：Complete
- 页码：1-8
- 全文大小：1480 K
文摘
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Rice starch synthase (SS) isozymes show poor activities toward linear dextrin.
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SSI specifically elongates the DP6 and 7 chains to form mainly the DP8 chain.
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SSI and BE specifically interact by activating the mutual capacities each other.
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The role of BE is not merely to supply SSI with increased primers.
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The addition of citrate is absolutely required for the SSI–BE interaction.