Sensitive determination of neurotransmitters in urine by microchip electrophoresis with multiple-concentration approaches combining field-amplified and reversed-field stacking
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- 作者：Yan Zhang ; Yi Zhang ; Guan Wang ; Wujuan Chen ; Yi Li ; Yating Zhang ; Pingang He ; Qingjiang Wang ; qjwang@chem.ecnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Microchip electrophoresis ; Neurotransmitters ; Field-amplified stacking ; Reversed-field stacking
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：1025
- 期：Complete
- 页码：33-39
- 全文大小：1358 K
文摘
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We combined field-amplified stacking (FASS) and reversed-field stacking (RFS) in a multiple-concentration approach for microchip electrophoresis detection of three neurotransmitters in human urine.
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Using this strategy, three neurotransmitters could be simultaneously focused and separated with high efficiency and sensitivity (LODs at S/N = 3 ranging from 1.69 to 2.73 nM).
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The multiple-concentration strategy afforded approximately 182- to 292-fold improvements in detection sensitivity.
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This strategy was successfully applied to three neurotransmitters in human urine.