A microchip electrophoresis-mass spectrometric platform with double cell lysis nano-electrodes for automated single cell analysis
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- 作者：Xiangtang Lia ; Shulin Zhaob ; Hankun Huc ; d ; Yi-Ming Liua ; d ; yiming.liu@jsums.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Microchip electrophoresis-mass spectrometry ; Single cell analysis ; Automation ; PC-12 cells ; Dopamine ; Glutamic acid
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：17 June 2016
- 年：2016
- 卷：1451
- 期：Complete
- 页码：156-163
- 全文大小：1762 K
文摘
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A microchip electrophoresis-mass spectrometric method is developed for analysis of single cells.
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Double nano-electrode cell lysis technique is deployed for the first time in single cell analysis.
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Intracellular levels of dopamine and glutamic acid in PC-12 neuronal cells are determined.
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KCl-induced changes in intracellular dopamine and glutamic acid levels are measured.