Study on sensing strategy and performance of a microfluidic chemiluminescence aptazyme sensor
详细信息 查看全文
- 作者：Qiwang Wua ; Haihui Shenb ; Hong Shena ; shzju@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yue Sunb ; Lifang Songa
- 关键词：CL ; chemiluminescence ; AO-DNA1 ; ssDNA composed of adenosine aptamer sequence and some other GO-DNA1 complementary bases for Sensor1 ; GO-DNA1 ; ssDNA composed of CatG4 DNAzyme sequence and some other AO-DNA1 complementary bases for Sensor1 ; AG-DNA2 ; ssDNA composed of concatenated adenosine aptamer sequence and CatG4 DNAzyme sequence for Sensor2 ; B-DNA2 ; ssDNA for AG-DNA2 blocking ; N-PAGE ; native polyacrylamide gel electrophoresis ; S/B ratio ; sample/background signal ratio ; FHS ; functional hairpin
- 刊名：Talanta
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：150
- 期：Complete
- 页码：531-538
- 全文大小：4422 K
文摘
- •
Sensing strategy of aptasensor is studied on thermodynamic equilibrium consideration.
- •
Both oligonucleotide arrangement and cation condition influence the S/B signal ratio.
- •
Low LOD and small sample consumption achieved by using microfluidic CL assay.