We identified the major transcription start site of the porcine PADI6 gene.
We identified a 153 bp minimal core promoter drove the basal expression of PADI6.
Transcription factor Sp1 bound to the − 56/− 47 region of the minimal promoter.
Overexpression of Sp1 significantly increased the promoter activity of PADI6 gene.
Inhibition of Sp1 significantly reduced the promoter activity of PADI6 gene.